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Table 2 Elution positions of RGP, KGP, and some other enzymes in the gel filtration on Sephacryl S-300

From: Cellular locations of proteinases and association with vesicles in porphyromonas gingivalis

  void volume valid volume
RGP   
crude extract + +
culture supernatant - +
vesicle extract + tr
envelope extract + +
KGP   
crude extract + +
culture supernatant - +
vesicle extract + +
envelope extract + +
Glycyl-phenylalaninyl-pna (dPP 1)  
crude extract - +
culture supernatant - +
vesicle extract - +
envelope extract - +
Lysyl-alanyl-pna (dPP 2)   
crude extract - tr
culture supernatant - -
vesicle extract - -
envelope extract - -
Glycyl-prolyl-pna (dPP 4)   
crude extract - +
culture supernatant - -
vesicle extract - -
envelope extract - tr
alanyl-alanyl-prolyl-pna   
crude extract - +
culture supernatant - -
vesicle extract - tr
envelope extract - tr
Alkaline phosphatase   
crude extract - +
culture supernatant - +
vesicle extract - +
envelope extract - +
  1. tr; trace