Table 2 Elution positions of RGP, KGP, and some other enzymes in the gel filtration on Sephacryl S-300
From: Cellular locations of proteinases and association with vesicles in porphyromonas gingivalis
| Â | void volume | valid volume |
|---|---|---|
RGP | Â | Â |
crude extract | + | + |
culture supernatant | - | + |
vesicle extract | + | tr |
envelope extract | + | + |
KGP | Â | Â |
crude extract | + | + |
culture supernatant | - | + |
vesicle extract | + | + |
envelope extract | + | + |
Glycyl-phenylalaninyl-pna (dPP 1) | Â | |
crude extract | - | + |
culture supernatant | - | + |
vesicle extract | - | + |
envelope extract | - | + |
Lysyl-alanyl-pna (dPP 2) | Â | Â |
crude extract | - | tr |
culture supernatant | - | - |
vesicle extract | - | - |
envelope extract | - | - |
Glycyl-prolyl-pna (dPP 4) | Â | Â |
crude extract | - | + |
culture supernatant | - | - |
vesicle extract | - | - |
envelope extract | - | tr |
alanyl-alanyl-prolyl-pna | Â | Â |
crude extract | - | + |
culture supernatant | - | - |
vesicle extract | - | tr |
envelope extract | - | tr |
Alkaline phosphatase | Â | Â |
crude extract | - | + |
culture supernatant | - | + |
vesicle extract | - | + |
envelope extract | - | + |