Table 1 Sequences of PCR primers, amplicon sizes, temperatures and GC content in SNPs of ZBTB17, HSPB7 , and ACTC1 in DCM patients and control subjects
Markers | Forward primer (5′-3′) | Reverse primer (5′-3′) | Amplicon size (bp) | Temperature (°C) | GC (%) |
|---|---|---|---|---|---|
ZBTB17 | |||||
rs10927875 | GCCAGAGTGGATGATCACTG | TTCTGTTTCCACCACTGTAG | 91 | 54.2 | 55 |
HSPB7 | |||||
rs1739843 | TGTCCTCACTCTGCCATCAC | TGGGCAGAGGGAGCCTGAG | 99 | 50.8 | 50 |
rs7523558 | GCCCAGCACCTATTTATAGC | CTCATAGGCCAGTGATGAAG | 94 | 46.6 | 35 |
rs6660685 | TACTGTCCCACAGCCAGCAC | TCTTCTCAGCCTCCTGGTG | 98 | 50.8 | 58.8 |
ACTC1 | |||||
rs533021 | GGCCTTCCATTTGAATATGC | GTATGAGAGCACATTTTCTG | 96 | 45.1 | 33.3 |
rs589759 | CTTCTCACCTCCCTAATTCC | TGTGGCCTGGAGGCTTTAAG | 96 | 49.4 | 45 |
rs1370154 | GCAGCAACTCATTCTAGAG | TTTCATACCTGAAAAGCAG | 108 | 45.2 | 24 |
rs2070664 | CATAACAATGACTGCTGCAC | ATAGCTTGTGGAGATAGGTC | 100 | 46.9 | 60 |
rs3759834 | TCTCCATCAAAGTATTTGCC | GATGAGCATCTTTAAACTGG | 100 | 52 | 45 |
rs525720 | GTTCTTGACTGGAGCTTTG | GCCAGTGCCCAGTTTTCTAT | 103 | 45.6 | 33.3 |
rs670957 | ATGCCAAGAGTAGAACTGCC | TCTGACACCAGTCCCATTTC | 97 | 45.5 | 50 |