Figure 3

Cells were cultured in Transwell chambers with the upper part coated with laminin (LN) or fibronectin (FN) respectively for 48 hours. The invaded cells were numerated under a light microscope (×400). (A) HepG2 infected by control shRNA plasmids invaded LN. (B) HepG2 cells infected by control shRNA plasmids invaded FN. (C) HepG2 cells infected by integrin α6 shRNA plasmids invaded LN. HepG2 had a decreased invasion potential after the knockdown of integrin α6 with significant statistical difference (P < 0.0001). It also proved that LN can increase invasion potential of HepG2 (P < 0.05).