Figure 5
From: Paris saponin VII suppressed the growth of human cervical cancer Hela cells
Activation of caspase-3 and caspase-9 after treatment with PS VII. Cell lysates were prepared after treatment with drugs for 24 h and then analyzed by Western blot with caspase-3 or caspase-9 antibody. The protein level of cleaved caspase-3 and caspase-9 was increased by PS VII treatment. However, the activation of caspase-3 and caspase-9 was minimized after pretreatment of a pan-caspase inhibitor (Z-VAD-FMK). ‘Quantity one’ of Bio-Rad and SPSS 17.0 were used to deal with the results of Western blot. *P < 0.05 vs. control; **P < 0.01 vs. control.