Association of methylenetetrahytrofolate reductase (MTHFR) C677T and A1298C polymorphisms with the susceptibility of childhood acute lymphoblastic leukaemia (ALL) in Chinese population

Table 1 Characteristics of PCR primers sequence, target fragment length and annealing temperature used for genotyping MTHFR polymorphisms

Polymorphisms sites		Primers sequence (5′ → 3′)	Fragment length	Annealing temperature
MTHFR C677T	F	AGTCCCTGTGGTCTCTTCATC	387 bp	56°C
MTHFR C677T	R	GGAGATCTGGGAAGAACTCAG	387 bp	56°C
MTHFR A1298C	F	CTTTGGGGAGCTGAAGGACTACTAC	168 bp	55°C
MTHFR A1298C	R	CACTTTGTGACCATTCCGGTTTG	168 bp	55°C

ISSN: 2047-783X