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Figure 1 | European Journal of Medical Research

Figure 1

From: Atomic force measurements on the specifically orientated membrane protein TGR5 reconstituted inside a tethered bilayer lipid membrane

Figure 1

Coupling Steps & Incorporation of protein into artificial membrane To reconstitute a membrane protein into a tBLM in a defined orientation we use the following approach. A PEG linker is coupled to a surface functionalized with amino-groups (1). To the PEG linker's free amino-group the anchor lipid, 1,2-dipalmitoyl-sn-glycero-3-phosphoethanol-amine-N-(succinyl) sodium salt and trisNTA are bound (2). Through the proteins His6-tag the protein is bound onto the surface with a defined orientation (3). Finally a complete membrane is formed with the free lipid 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (4).

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European Journal of Medical Research

ISSN: 2047-783X

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