Immunofluorescent staining to determine the localization of AT1 and AT2 in basal and apical portions of the experimental group seven days after vagal stimulation. A: Immunofluorescent staining of angiotensin subtype-1 (AT1) (green) and nucleus (blue) in the myocardium. B: Immunofluorescent staining of angiotensin angiotensin subtype-2 (AT2) (green) and nucleus (blue) in the myocardium. Results are from a single experiment but representative of three separate experiments. All bars represent 50 μm.