Figure 5
From: Proteomic analysis of exosomes derived from human lymphoma cells
The presence of actin, HSP70 (a), and ICAM-1 (b) is confirmed by Western blot analysis of EXO Raji . The primary antibodies were anti-actin (1:10,000) and anti-HSP70 (1:1,000), followed by horseradish peroxidase-conjugated goat anti-mouse IgG1. Blots were incubated with SuperSignal West Pico chemiluminescent substrate and exposed to Hyperfilm ECL (Amersham Biosciences, Shanghai, China).