Figure 4

Relationship between ID4 methylation levels and BM blasts during disease status in five MDS patient samples at different disease stages. ID4 gene methylation levels increased or decreased with the number of bone marrow blasts. (A) ID4 methylation level of one patient during different disease stages MDS-RA, MDS-RAEB, AML, day 37 to day 200 after transplantation. (B) ID4 methylation level of four patients in different disease stages. (C) BM blasts of four patients in different disease stages. Stage 1: new diagnosis; stage 2: the first period of demethylation treatment for patient 2 and patient 3 and 1 month after transplantation for patient 4 and patient 5; stage 3: the second period of demethylation treatment for patient 2 and patient 3 and 2 months after transplantation for patient 4 and patient 5. AML, acute myeloid leukemia; ID4, inhibitor of DNA binding 4; MDS-RA, MDS with refractory anemia; MDS-RAEB, MDS with refractory anemia with excess blasts.