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Table 2 Sequences of the primers and probes

From: Clinical implications of the quantitative detection of ID4 gene methylation in myelodysplastic syndrome

  Forward Reverse
BSP   
ID4 primer 5′-GTTTGATTGGTTGGTTATTTTAGAT-3′ 5′-ACCGAAAAAAAAATAACCCAC-3′
5′-CACCAAAAAAAAAATAACCCAC-3′
Methylight PCR   
MYOD1 primer 5′-CCAACTCCAAATCCCCTCTCTAT-3′ 5′-TGATTAATTTAGATTGGGTTTAGAGAAGGA-3′
MYOD1 probe 5′-TCCCTTCCTATTCCTAAATCCAACCTAAATACCTCC-3′  
ID4 primer 5′-TCGGAGTTTTCGTTTTCGTT-3′ 5′-CGATACTACTCACAACCGCG-3′
ID4 probe 5′-AGCGGGTTTCGTTCGGTTCG-3′  
  1. ID4, inhibitor of DNA binding 4; BSP, bisulfite sequencing PCR.