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Table 2 Sequences of the primers and probes

From: Clinical implications of the quantitative detection of ID4 gene methylation in myelodysplastic syndrome

 

Forward

Reverse

BSP

  

ID4 primer

5′-GTTTGATTGGTTGGTTATTTTAGAT-3′

5′-ACCGAAAAAAAAATAACCCAC-3′

5′-CACCAAAAAAAAAATAACCCAC-3′

Methylight PCR

  

MYOD1 primer

5′-CCAACTCCAAATCCCCTCTCTAT-3′

5′-TGATTAATTTAGATTGGGTTTAGAGAAGGA-3′

MYOD1 probe

5′-TCCCTTCCTATTCCTAAATCCAACCTAAATACCTCC-3′

 

ID4 primer

5′-TCGGAGTTTTCGTTTTCGTT-3′

5′-CGATACTACTCACAACCGCG-3′

ID4 probe

5′-AGCGGGTTTCGTTCGGTTCG-3′

 
  1. ID4, inhibitor of DNA binding 4; BSP, bisulfite sequencing PCR.