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Table 1 Expression of virulence gene ply after S. pneumoniae infected THP-1 or A549 cells

From: In vitro expression of Streptococcus pneumoniae ply gene in human monocytes and pneumocytes

Groups n ply (Ct,‾x ± s )
   0 h 4 h (THP-1) 8 h (THP-1) 4 h (A549) 8 h (A549)
ATCC 1 16.2 31.5 27.9 21.8 19.1
Blood SP 11 29.2 ± 2.6 24.8 ± 3.2 23.0 ± 1.5 20.4 ± 5.1 18.2 ± 2.9
Sputum SP 12 28.1 ± 2.9 26.3 ± 2.7 24.6 ± 1.4 20.8 ± 3.0 23.3 ± 3.1
  1. Note: Ct = Mean Ct of tested gene − Mean Ct of control gene. Smaller Ct means more ply expression. Level of 16S rRNA was used as a control, which expresses much higher than that of ply. SP, Streptococcus pneumoniae; ATCC, American Type Culture Collection.