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Table 1 Expression of virulence gene ply after S. pneumoniae infected THP-1 or A549 cells

From: In vitro expression of Streptococcus pneumoniae ply gene in human monocytes and pneumocytes

Groups

n

ply (△Ct,‾x ± s )

  

0 h

4 h (THP-1)

8 h (THP-1)

4 h (A549)

8 h (A549)

ATCC

1

16.2

31.5

27.9

21.8

19.1

Blood SP

11

29.2 ± 2.6

24.8 ± 3.2

23.0 ± 1.5

20.4 ± 5.1

18.2 ± 2.9

Sputum SP

12

28.1 ± 2.9

26.3 ± 2.7

24.6 ± 1.4

20.8 ± 3.0

23.3 ± 3.1

  1. Note: △Ct = Mean Ct of tested gene − Mean Ct of control gene. Smaller △Ct means more ply expression. Level of 16S rRNA was used as a control, which expresses much higher than that of ply. SP, Streptococcus pneumoniae; ATCC, American Type Culture Collection.