Fig. 2

Photographs of the important steps of the isolation process a solid fat from abdominoplasty b solid fat cut into small pieces (at a range of 1–3 mm) by using a scalpel and forceps c transferring 10–15 ml of minced fat into a 50 ml reaction tube d adding DPBS up to a total volume of 50 ml e transferring of the upper fat layer into a new reaction tube f adding an equal volume of collagenase solution (0.5 mg/ml in DPBS; 355 U/mg) through a filter g mixing the minced fat with the collagenase solution h appearance of the mixture of fat tissue and collagenase just before incubation at 37 °C with pieces of fat tissue visible i homogeneous emulsion without remaining pieces of fat tissue after incubation at 37 °C in a water bath j discarding the upper fat layer and removing the supernatant after centrifugation k filtrating the suspension through a cell strainer (40 µm) into a fresh reaction tube