Fig. 1

Protein elution kinetics during the decellularization process. Native rat kidneys were perfused with different concentrations of SDS solution. Flowout of SDS solution from kidneys was collected at 5-min intervals and investigated for protein content using BCA assay. Most protein is eluted within the first 5 min of perfusion. Protein levels are decreasing over time. After 30 min of perfusion, detectable protein concentrations are still eluted from the tissue. n = 1