Fig. 3

Integrity of the extracellular matrix after decellularization procedures. Upper pictures Allura Red dye injection into the renal artery. Medial pictures immunohistochemic staining for Laminin. Lower pictures immunohistochemic staining for Fibronectin. After decellularization with 0.66% SDS/60 min (left column) vascular network remains mainly preserved, as the vascular leakage is greater after the harshest decellularization protocol with 1% SDS/120 min (right column). Stainings for Laminin and Fibronectin reveal that the general matrix architecture is mainly preserved, even after using the harshest decellularization protocol (1% SDS/120 min, right column). Representative pictures. Black bars indicate 100 µm. n = 3