Fig. 4

Activation of the Wnt signaling pathway promoted hUCMSCs survival on the CCLDADM scaffold. a hUCMSCs were seeded on the CCLDADM scaffold and treated with Wnt3a or sFRP3 for 72 h, followed by DAPI staining to estimate the total number of cells on the scaffold (scale: 200 μm). b CCK-8 staining detected the total number of cells on the CCLDADM scaffold at 24 or 72 h post-drug intervention (n = 3). c hUCMSCs were seeded in a 6-well plate and treated for 72 h with Wnt3a or sFRP3, and the ratio of BrdU-positive cells was detected to reflect the rate of proliferation (scale: 100 μm). d Statistical analysis of BrdU-positive ratio from c (n = 3). e Live/dead cell staining evaluated the ratio of dead cells on the CCLDADM scaffold (scale: 100 μm). f Statistical analysis of results from e (n = 3); *P < 0.05, **P < 0.01 vs. control