Fig. 3

LPS induced the injury and inflammatory factors expression of cardiomyocytes H9c2 cells. a The cell viability of H9c2 cells was analyzed with CCK-8 assay. b Flow cytometry was applied to observe the apoptotic cells after staining with Annexin V-FITC/PI. c Western blot assay was performed to examine the expression of apoptosis-associated proteins. d The mRNA expression of MCP-1 and IL-6 was quantified with qRT-PCR assay. e ELISA was conducted to quantify the concentration of MCP-1 and IL-6. f The protein expression of MCP-1 and IL-6 was analyzed with Western blot assay. H9c2 cells were stimulated with 10 μg/mL LPS for 6 h. **P < 0.01 or ***P < 0.001. Data were presented as mean ± standard deviation (SD) of at least three independent experiments. LPS lipopolysaccharide, MCP-1 monocyte chemo-attractant protein-1, IL-6 interleukin-6, CCK-8 cell counting kit-8, Annexin V-FITC/PI Annexin V-fluorescein isothiocyanate/propidium iodide, qRT-PCR quantitative real-time PCR, ELISA enzyme-linked immune sorbent assay