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Table 1 PCR primers (in the 5′ – 3′ direction)

From: Fracture fixation strategy and specific muscle tissue availability of neutrophilic granulocytes following mono- and polytrauma: intramedullary nailing vs. external fixation of femoral fractures

Primer

Sequence 5′-3′

Sus scrofa domesticus

 

β-Actin forward

GGACTTCGAGCAGGAGATGG

β-Actin reverse

GCACCGTGTTGGCGTAGAGG

RSP 18 forward

GGGTGTAGGACGGAGATAT

RSP 18 reverse

ATTACACGTTCCACCTCATC

HPRT forward

GTCAAGCAGCATAATCCAAAG

HPRT reverse

AAGGGCATAGCCTACCACAA

PPIA forward

AGCACTGGGGAGAAAGGATT

PPIA reverse

AAAACTGGGAACCGTTTG

Interleukin 6 forward

GAATCCAGACAAAGCCACCA

Interleukin 6 reverse

GTGCCCCAGCTACATTATCC

Interleukin 8 forward

ACTGCTGTTGTTGTTGCTTC

Interleukin 8 reverse

ATATCTGTACAACCTTCTGC

  1. The PCR was run with the specified primers on a StepOne Plus RT device