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Fig. 5 | European Journal of Medical Research

Fig. 5

From: Thrombin-activated platelet-rich plasma enhances osteogenic differentiation of human periodontal ligament stem cells by activating SIRT1-mediated autophagy

Fig. 5

Effects of autophagy inhibition on activated PRP-induced hPDLSC osteogenic differentiation. hPDLSCs were treated with 20% thrombin-activated PRP or together with sirtinol or 3-MA. A ARS staining was performed to examine calcium deposits. B QRT-PCR results showed the expression of COL-I, OPN, Runx2 and OCN. GAPDH was used as an internal control. ***P < 0.001

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European Journal of Medical Research

ISSN: 2047-783X

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