Fig. 2

The effects of HG treatment on HK-2 cell proliferation, oxidative stress and apoptosis. A-K HK-2 cells were treated with 30 mmol/L D-glucose, 5 mmol/L D-glucose and 30 mmol/L Mannitol, respectively, and cell viability was analyzed by a CCK-8 assay (A), cell proliferation by an EdU assay (B), ROS level by a cellular ROS assay kit (C), MDA production by a lipid peroxidation MDA assay kit (D), SOD activity by a Superoxide dismutase activity assay kit (E), caspase 3 activity by a caspase 3 activity assay (F), cell apoptotic rate by flow cytometry analysis (G), and the protein expression of Bax, cleaved caspase 3 and Bcl 2 by Western blotting analysis (H–K). ***P < 0.001