Fig. 3

miR‑199a/b-3p was the target of PAK4 and BCAR3. A TargetScan and DIANA database were used to predict the target miRNAs of PAK4 and BCAR3. B Sequences of predicted miR-199a/b-3p binding sites within PAK4 3’-UTR in DIANA database. C Sequences of predicted miR-199a/b-3p binding sites within BCAR3 3’-UTR in DIANA database. D, E Effect of miR-199a/b-3p on luciferase intensity controlled by the wild type (WT) or mutant (MUT) 3’UTR of PAK4/BCAR3 was determined using the luciferase assay; **p < 0.01. F, G mRNA levels of PAK4/BCAR3 were detected using quantitative RT-PCR in SW1116 cells transfected with miR-199a/b-3p mimics; **p < 0.01. H Protein levels of PAK4 and BCAR3 were detected using western blot in SW1116 cells transfected with miR-199a/b-3p mimics