Fig. 1
From: GM-CSF-mediated inducement of bone marrow MDSCs by TSA and effect on survival of graft in mice
TSA can promote the GM-CSF-induced BM cell differentiation and increase the production of CD11b + Gr-1 + MDSCs. A TSA can promote the GM-CSF-induced BM cell differentiation. TSA was added to the culture medium containing 1000 u/ml GM-CSF to induce the differentiation of BM cells from B6 mice. Mean ± SD was obtained from 3 independent experimental results. * P < 0.05, compared to the 0 nM group; B TSA can promote the production of CD11b + Gr-1 + MDSCs. The three images, respectively, show the cells cultured with GM-CSF, GM-CSF + 10 nM TSA, and high-quality CD11b + Gr-1 + MDSCs sorted by flow cytometry. Cell phenotype was analyzed in flow cytometry. Each experiment was performed in triplicate