Fig. 2
From: GM-CSF-mediated inducement of bone marrow MDSCs by TSA and effect on survival of graft in mice
The MDSCs induced by TSA + GM-CSF has immunosuppressive activity. A Western blot showed decreasing iNOS and HO-1 expression while increasing histone H4 acetylation level in GM-CSF-BM cells after addition of TSA. GAPDH was the reference. MDSCs were obtained by magnetic bead sorting from cells cultured with GM-CSF or 10 nM TSA-GM-CSF after 7 days; B iNOS and HO-1 are indispensable in the suppressive actions of GM-CSF/TSA-GM-CSF stimulated MDSCs for T cell proliferation. The number of MDSCs was 3 × 104. iNOS inhibitor L-NMMA and HO-1 inhibitor SnPP were added to culture medium from day 0. Each experiment was performed in triplicate