Table 1 Gene-specific primers used for real-time RT-PCR
Gene | Forward primer | Reverse primer |
|---|---|---|
TNF-Α | 5ʹ-AGATGTGGAACTGGCAGAGG-3ʹ | 5ʹ-CACGAGCAGGAATGAGAAGAG-3ʹ |
IL-1Β | 5ʹ-CTCGTGGGATGATGACGACC-3ʹ | 5ʹ-AGGCCACAGGGATTTTGTCG-3ʹ |
IL-6 | 5ʹ-TCCTACCCCAACTTCCAATGC-3ʹ | 5ʹ-GGTTTGCCGAGTAGACCTCAT-3ʹ |
IL-17A | 5ʹ-CGCCGAGGCCAATAACTTTC-3ʹ | 5ʹ-GGTTGAGGTAGTCTGAGGGC-3ʹ |
IFN-Γ | 5ʹ-GGCAAAGGACGGTAACACG-3ʹ | 5ʹ-TCTGTGGGTTGTTCACCTCG-3ʹ |
Β-Actin | 5ʹ-CACCCGCGAGTACAACCTTC-3ʹ | 5ʹ-CCCATACCCACCATCACACC-3ʹ |