Fig. 6

STAT3 inhibition weakened the damaging function of miR-155-5p overexpression in MLE-12 cells. MLE-12 cells were transfected along with miR-155-5p mimics, and then the STAT3 inhibitor Stattic was administered. A qRT-PCR confirmed miR-155-5p expression in MLE-12 cells. B, C BrdU and CCK8 measured MLE-12 viability and proliferation. D Flow cytometry tracked MLE-12 apoptosis. E Western blot determined the profiles of apoptosis-associated proteins in MLE-12 cells. F, G ELISA ascertained the profiles of TNF-α, IL-6, IL-1β, and IL-10 in MLE-12 cells. H Western blot gauged the profile of SOCS5/JAK2/STAT3 in MLE-12 cells. **P < 0.01, ***P < 0.001, ****P < 0.0001 (vs. the control group); NS P > 0.05, &&P < 0.01, &&&P < 0.001 (vs. the miR-155-5p group). N = 3