Table 1 Reverse and forward primers used in this study for PCR procedure
From: TCAP gene is not a common cause of cardiomyopathy in Iranian patients
Exon No. | Primer sequences 5′ to 3′ direction | Primer length | Product length | GC% | TM(˚c) |
|---|---|---|---|---|---|
1 Forward | ACTTATAGCATCTGACACCAGAGG | 24 bp | 956 | 45.8 | 69.6 |
1 Reverse | AAATTTCTCCAGGGCTTCATG | 21 bp | 956 | 42.9 | 72.1 |
2 Forward | TGAAGCCCTGGAGAAATTTCTG | 22 bp | 956 | 45.5 | 74.1 |
2 Reverse | GCAAACTACAAAGCAGCCATG | 20 bp | 956 | 47.6 | 72.2 |