Fig. 3

C3 deficiency inhibited TLR4 / NFκB-P65 signaling pathway activation during post-injury fibrosis. a Immunohistochemical detection of glomerular complement C3, TLR4, and NFκB-P65 protein expression (Scale bar = 25 μm) in each group of mice. b Renal tissue immunohistochemical staining optical density analysis, n = 10. c Western blot gel electrophoresis film exposure map, d Western blot gel electrophoresis exposure film grayscale analysis, with the target protein/β-actin (OD) ratio to express the target protein expression level, n = 10. **P < 0.01vs WT-sham or C3^−/−-sham, ## P < 0.01vs WT-AKI -CKD