Fig. 5

Effect of tubular cell secretome on α-SMA expression in renal fibroblasts. After 24-h incubation with or without secretome derived from control or COM-treated HK-2 or MDCK cells, α-SMA expression in BHK-21 cells was examined by immunofluorescence staining using mouse monoclonal anti-α-SMA primary antibody and Alexa Flour 488-conjugated anti-mouse IgG secondary antibody. (A) and (C): Immunofluorescence micrographs of α-SMA (in green) and nuclei (in blue) in BHK-21 cells treated with secretome derived from HK-2 and MDCK cells, respectively. (B) and (D): Fluorescence intensity data were analyzed from at least 100 cells in ≥ 10 random high-power fields (HPFs) per each sample. Each bar represents mean ± SD of measurements in each dataset (derived from three independent experiments using different biological replicates). Only significant p values are labeled. AU arbitrary unit