Fig. 2

The identification of “stiff tumor” and “soft tumor”. A. Venn plot showing the intersection of DEGs and membrane tension-related genes; B. identifying 8 genes using Lasso regression analysis; C. lasso coefficient profiles of the identifying genes; D. the results of heatmaps using NMF algorithm in TCGA cohort; E. two subtypes were identified the optimal value using NMF algorithm in MSKCC2010 cohort; F. two subtypes were identified the optimal value using NMF algorithm in GSE116918 dataset; G. two subtypes were identified the optimal value using NMF algorithm in GSE46602 dataset; H. Kaplan–Meier curve showing the survival differences between two subtypes in TCGA cohort; I. Kaplan–Meier curve showing the survival differences between two subtypes in MSKCC2010 cohort; J. Kaplan–Meier curve showing the survival differences between two subtypes in GSE116918 dataset; K. Kaplan–Meier curve showing the survival differences between two subtypes in GSE46602 dataset. DEGs = differentially expressed genes; NMF = nonnegative matrix factorization; TCGA = the cancer genome atlas; BCR = biochemical recurrence; GEO = gene expression omnibus