Fig. 6

SchA Treatment Upregulates the Nrf2 Pathway in CS-induced COPD Model Mice. A The protein expression levels of Nrf2 and HO-1 were measured by Western blotting. β-Actin was used as the internal reference. B The band intensities of Nrf2 and HO-1 were semiquantified using ImageJ software. C Detection of Nrf2 levels in lung tissue by immunohistochemistry (scale bar = 100 μm). D Quantitative analysis statistics of Nrf2 in immunohistochemistry. n = 3 mice per group (A, B), n = 6 mice per group (C, D). Data are shown as the mean ± SEM (B, D). To test for group differences, Games–Howell (B Nrf2/β-Actin) and Tukey (B HO-1/β-Actin, D). *p < 0.05, **p < 0.01, ***p < 0.001 versus control group, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 versus CS group, ⁺p < 0.05, ⁺⁺p < 0.01, ⁺⁺p < 0.001 versus CS + DEX group, ^§p < 0.05, ^§§p < 0.01, ^§§§p < 0.001 versus CS + SchA group