Fig. 7

SchA Treatment Inhibits NLRP3 Pathways in CS-induced COPD Model Mice. A The protein expression levels of NLRP3, ASC, pro-Caspase1, cleaved-Caspase1 IL-1β, IL-18, GSDMD and GSDMD-N were measured by Western blotting. β-Actin was used as the internal reference. B The band intensities of NLRP3, ASC, pro-Caspase1, cleaved-Caspase1 IL-1β, IL-18, GSDMD and GSDMD-N were semiquantified using ImageJ software. C Detection of NLRP3 levels in lung tissue by immunohistochemistry. D Quantitative analysis statistics of NLRP3 in immunohistochemistry. n = 3 mice per group (A–C), n = 6 mice per group (D, E). Data are shown as the mean ± SEM (B, C, E). To test for group differences, Games–Howell (E) and Tukey (B, C) tests were used. *p < 0.05, **p < 0.01, ***p < 0.001 versus control group, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 versus CS group, ⁺p < 0.05, ⁺⁺p < 0.01, ⁺⁺p < 0.001 versus CS + DEX group, ^§p < 0.05, ^§§p < 0.01, ^§§§p < 0.001 versus CS + SchA group