Table 1 The sequences of primers
Number | Gene | Accession numbers | Primer sequence (5'-3') | PCR product (bp) |
|---|---|---|---|---|
1 | CTLA-4 | NM_001281976.1 | F: CTCTGAAGCCATACAGGTGA | 200 |
R:GGTAATCTAGGAAGCCCACTG | ||||
2 | PD-1 | NM_008798.3 | F: GTGGCATCTACCTCTGTGG | 277 |
R: GTGTCGTCCTTGCTTCCAGC | ||||
3 | TIM-3 | NM_134250.2 | F: TCTCCAAGAACCCTAACCAC | 160 |
R: CAGAGACTCCCACTCCAATG | ||||
4 | LAG-3 | NM_008479.2 | F: CTCAATGCCACTGTCACG | 180 |
R: CTCCTGAATCTCCAGCACAG | ||||
5 | GAPDH | NM_001289726.1 | F: CACTGCCACCCAGAAGACTG | 147 |
R: CCAGTGAGCTTCCCGTTCAG | ||||
6 | T-bet | NM_019507.2 | F: TCAACCAGCACCAGACAGA | 109 |
R: AACATCCTGTAATGGCTTGT | ||||
7 | P53 | NM_011640.3 | F: GTATTTCACCCTCAAGATCC | 84 |
R: TGGGCATCCTTTAACTCTA | ||||
8 | Caspase-3 | NM_001284409.1 | F: CTCGCTCTGGTACGGATGTG | 201 |
R:TCCCATAAATGACCCCTTCAT |