Fig. 5

Three cuproptosis subtypes with distinct immunotherapeutic responses. A The relative activity status of the 50 hallmark pathways in the three cuproptosis subtypes in TCGA cohort. Colors from blue to red denote low to high activity. B Landscape of immune score, and stromal score, mRNA expression of common immune checkpoints, relative abundance of 22 tumor-infiltrating immune cell types and two stromal components in TCGA cohort. C Multi-omics profiling (mRNA expression, DNA methylation level, and amplification/deletion frequency) of 75 immunomodulators in TCGA cohort. D SubMap analysis shows the expression similarity between the three cuproptosis subtypes and responses to anti-PD-1 and anti-CTLA4 in TCGA cohort. E Validation of the expression similarity between the three cuproptosis subtypes and responses to anti-PD-1 and anti-CTLA4 in the E-MTAB-1980 dataset