Fig. 2

Effects of cisplatin treatment on apoptosis of GCs in mice. A Cleaved caspase-3 staining was analyzed by immunohistochemistry in the control and POF groups. Cleaved caspase-3 positive cells are shown as brown with the cell nucleus stained blue. B Apoptosis of GCs in ovarian tissues examined by TUNEL analysis. The red staining indicates FSHR-positive GCs. The green stain indicates TUNEL-positive GCs. The blue DAPI stain indicates the cell nucleus. Control group, n = 10; POF group, n = 10. GCs: granulosa cells; POF: premature ovarian failure; TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP–biotin nick end labeling; DAPI: 4′6-diamidino-2-phenylindole. *P < 0.05; **P < 0.01, two-tailed Student’s t test. Scale bars represent 50 μm (A), and 100 μm (B)