Fig. 3

Effects of cisplatin treatment on ovarian follicle activation in mice. A H&E staining displaying ovarian morphology and columns representing the proportion of follicles at various stages of development between the control group and POF group. B Protein levels of AKT, p-AKT, FoxO3A, and p-FoxO3A in the ovaries of the two groups. C Images of immunofluorescence showing the localization of p-FoxO3A using anti-p-FoxO3A antibodies (green) in the two groups. The primordial follicles were detected using an anti-LHX8 antibody (red). All nuclei were stained with DAPI. Control group, n = 10; POF group, n = 10. H&E, hematoxylin and erosion; POF, premature ovarian failure; DAPI, 4′6-diamidino-2-phenylindole. *P < 0.05; **P < 0.01, two-tailed Student’s t test. Scale bars represent 200 μm, 100 μm (A), and 10 μm (C)