Fig. 4

miRNA microarray analysis was performed to detect differentially expressed miRNAs in GCs of mice. A Identification of GCs. Isolated GCs show a spindle-shaped morphology. The red staining indicates FSHR-positive GCs. The blue DAPI stain indicates the GC nuclei. B miRNA expression in the POF group and the control group from three biological replicate samples was profiled using microRNA array. C Real-time PCR was used to verify the microarray results. D Fluorescence in situ hybridization detected expression of miR-144-3p in the control group and POF group. Control group, n = 10; POF group, n = 10. GCs: granulosa cells; FSHR: follicle-stimulating hormone receptor; DAPI: 4′6-diamidino-2-phenylindole; POF: premature ovarian failure. *P < 0.05; **P < 0.01; *** P < 0.001, two-tailed Student’s t test. Scale bar, 100 μm