Fig. 5

Map3k9 is upregulated in GCs of POF mice. A Bioinformatics prediction combined with mRNA microarray analysis were performed to identify the potential target genes of miR-144-3p. B Differentially expressed gene pathway enrichment analysis in the POF group and the control group. C Real-time PCR was used to evaluate the relative expression of target genes of miR-144-3p in the POF group and the control group. D Protein levels of MAP3K9 in both groups were detected using western blotting. E Immunohistochemistry analysis of the protein expression of MAP3K9 in both groups. Control group, n = 10; POF group, n = 10. GCs: granulosa cells; POF: premature ovarian failure. *P < 0.05; **P < 0.01, two-tailed Student’s t test. Scale bar, 100 μm