Fig. 6

MAP3K9 is a target of miR-144-3p. A Real-time PCR showing that miR-144-3p expression was significantly decreased in cisplatin injured COV434 cells. B MAP3K9 mRNA and protein levels increased significantly, as analyzed using real-time PCR and western blotting, respectively. C Real-time PCR and western blotting were performed to detect the mRNA and protein levels of MAP3K9 in the injured COV434 cells transfected with miR-144-3p agomir and antagomir. D Predicted target sites and relevant mutation sequences are shown. Luciferase reporter assay results demonstrating that overexpression of miR-144-3p could reduce the site-specific luciferase activity of the MAP3K9 gene with wild type 3′ untranslated region (UTR). *P < 0.05; **P < 0.01, two-tailed Student’s t test