Fig. 5
NOX1 promotes polarization of M0- to M2-type macrophages by stimulating ROS production. A Electron microscopy analysis of cell morphology. B Flow cytometry detection of CD11b and CD14 expression. C Fluorescence confocal microscopy for evaluation of exosomal uptake in M0 macrophages. D Fluorescence microscopy for evaluation of ROS production. E Flow cytometry detection of polarization of macrophages. Scale bar, 50 µm. Con, control; NC, negative control; OE, NOX1 overexpression; Ace, Acetylcysteine; exo, exosome