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Fig. 5 | European Journal of Medical Research

Fig. 5

From: Impaired autophagy contributes to the aggravated deterioration of osteoarthritis articular cartilage by peroxisome proliferator-activated receptor α deficiency, associated with decreased ERK and Akt activation

Fig. 5

Effect of PPARα activation by WY14643 on the expression of ECM and autophagy-related signal molecules in IL-1β-treated human OA chondrocytes. Cells were pretreated with WY14643 (50 μM), CQ (10 μM) or WY14643 (50 μM) + CQ (10 μM) for 2 h, respectively, and were then co-treated with IL-1β (10 ng/ml) for 22 h. A Expression of Collagen II, Aggrecan, MMP13, ADAMTS5, P62, LC3B, Beclin1, Bcl2, and β-actin protein levels were detected via western blotting; B GAG levels were measured via Dimethylmethylene blue assay; C autophagic vacuoles were observed under a transmission electron microscope; D quantities of autophagic vacuoles. The data are representative of three independent experiments (*p < 0.05)

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European Journal of Medical Research

ISSN: 2047-783X

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