Fig. 1

Abnormally high expression of circCOL5A1 in RCC. A Heat map of differential expression of circRNAs in normal tissue and RCC tissue; B volcanic maps of different circRNAs; C top 10 circRNAs with highest expression and lowest expression; D changes of circCOL5A1 expression; E genetic information of circCOL5A1; F circCOL5A1 in RCC tissues and normal tissues analyzed by RT-qPCR. G circCOL5A1 in HK-2 and human RCC cell lines analyzed by RT-qPCR; H, I the stability of circCOL5A1 evaluated by actinomycin D assay and RNAse R experiment; J gel electrophoresis to examine the products of RT-qPCR amplification using polymeric and diverging primers; data were expressed as mean ± SD (N = 3). * P < 0.05