Fig. 2

circCOL5A1 knockdown impairs RCC progression. circCOL5A1 targeted siRNA was transfected into ACHN cells. A circCOL5A1 expression analyzed by RT-qPCR; B, C cell proliferation detected by clonal assay and EdU assay; D apoptosis evaluated by flow cytometry; E invasion and migration evaluated by Transwell assays; F–H glucose consumption, lactic acid production, and ATP levels; I HK2 and PKM2 detected by Western blot; data were expressed as mean ± SD (N = 3). * P < 0.05