Fig. 4

miR-370-5p is involved in the process of circCOL5A1 regulation of RCC. pcDNA 3.1-circCOL5A1 and miR-370-5p mimic were co-transfected into ACHN cells. A circCOL5A1 and miR-370-5p expression analyzed by RT-qPCR; B, C cell proliferation detected by clonal assay and EdU assay; D apoptosis evaluated by flow cytometry; E invasion and migration evaluated by Transwell assays; F–H glucose consumption, lactic acid production, and ATP levels; I HK2 and PKM2 detected by Western blot; Data were expressed as mean ± SD (N = 3). * P < 0.05