Fig. 5

Targeted regulation of PRKCSH by miR-370-5p. A RT-qPCR detection of mRNA expression after overexpressing miR-370-5p; B potential binding sites of miR-370-5p and PRKCSH; C, D the targeting relationship between miR-370-5p and PRKCSH detected by dual luciferase reporter assay and RIP experiment; E PRKCSH expression in RCC tissues and normal tissues analyzed by Western blot; F PRKCSH expression in HK-2 and human RCC cell lines analyzed by Western blot; data were expressed as mean ± SD (N = 3). * P < 0.05