Fig. 6

CircCOL5A1 affects RCC progression via miR-370-5p/PRKCSH axis. si-circCOL5A1 and pcDNA 3.1-PRKCSH were co-transfected into ACHN cells. A PRKCSH expression analyzed by Western blot; B, C cell proliferation detected by clonal assay and EdU assay; D apoptosis evaluated by flow cytometry; E invasion and migration evaluated by Transwell assays; F–H glucose consumption, lactic acid production, and ATP levels; I HK2 and PKM2 detected by Western blot; Data were expressed as mean ± SD (N = 3). * P < 0.05