Table 1 Gene sequence primers
Genes | Sequences (5ʹ–3ʹ) |
|---|---|
circCOL5A1 | Forward: 5ʹ-CCTAACCAAGGATGCTCCAGG-3ʹ |
Reverse: 5ʹ-GGCCCCCTTCGGACTTCT-3ʹ | |
miR-370-5p | Forward: 5ʹ-GCAGGTCACGTCTCTGC-3ʹ |
Reverse: 5ʹ-TGGTGTCGTGGAGTCG-3ʹ | |
PRKCSH | Forward: 5ʹ-TGCCTTCAAGGAGCTGGATG-3ʹ |
Reverse: 5ʹ-AAAGAGGTGGCGTCTGTCTG-3ʹ | |
U6 | Forward: 5ʹ-CTCGCTTCGGCAGCACA-3ʹ |
Reverse: 5ʹ-AACGCTTCACGAATTTGCGT-3ʹ | |
GAPDH | Forward: 5'-CACCCACTCCTCCACCTTTG-3' |
Reverse: 5ʹ-CCACCACCCTGTTGCTGTAG-3ʹ |