Fig. 3

MLN4924 enhances the cytotoxicity of 10-HCPT to HNSCC cells. A AMC-HN-8 and CAL-27 cells were treated with various concentrations of MLN4924 for 72 h, followed by ATP-lite assay. B Cells were treated with various concentrations of 10-HCPT alone or in combination with an IC₂₀ concentration of MLN4924, followed by ATP-lite assay. **p < 0.01, ***p < 0.001 (n = 3). C AMC-HN-8 cells were seeded in triplicate in 60-mm dishes at 350 cells per dish and treated with the indicated concentrations of MLN4924 (0, 25, 50 nM) alone or in combination with 10-HCPT (2.5 nM) for 7–14 days; Then, colonies were quantified. *p < 0.05, **p < 0.01 (n = 3)