Fig. 5

Combination of MLN4924 and 10-HCPT promotes apoptosis of HNSCC cells. A Cells were treated with MLN4924 (50 nM) alone or in combination with 10-HCPT (5 nM). Cell apoptosis was analyzed by flow cytometry (mean ± SEM, n = 3). B Cells were treated with various concentrations of MLN4924 (0, 0.25 µM) alone or in combination with 10-HCPT (0, 0.5, or 1 µM) for 24 h and then subjected to western blot analysis using the indicated antibodies