Fig. 3

CDDP and TA synergistically increased the apoptosis of H1299 cells. A The morphology of cells treated with CDDP or/and TA for 24 and 48 h was observed under a light microscope. The scale bar is 100 μm. B The nuclei of cells treated with CDDP and/or TA for 24 and 48 h were observed under a fluorescence microscope, and apoptotic bodies or nuclear debris were observed. The scale bar is 50 μm. C Flow cytometry analysis showed the percentage of necrotic cells, late apoptotic cells, viable cells, and early apoptotic cells (Denoted by quadrants D1, D2, D3, and D4)