Table 1 Primers of genes related to endoplasmic reticulum stress pathway used in experiments
Gene | Primer sequence (5′-3′) | Length (bp) |
|---|---|---|
ATF4 | F:ATGACCGAAATGAGCTTCCTG R:GCTGGAGAACCCATGAGGT | 153 |
ATF6 | F:TCCTCGGTCAGTGGACTCTTA R:CTTGGGCTGAATTGAAGGTTTTG | 235 |
Caspase3 | F:GAAATTGTGGAATTGATGCGTGA R:CTACAACGATCCCCTCTGAAAAA | 164 |
CHOP | F:CAAGAGGTCCTGTCTTCAGATGA R:TCTGTTTCCGTTTCCTGGTTC | 247 |
GAPDH | F:AAGCTAGTTACAAAAAGGCCATCATT R:AGGGTTCGGACTCCTGGAA | 45 |
GRP78 | F:TCAAGTTCTTGCCGTTCAAGG R:AAATAAGCCTCAGCGGTTTCTT | 148 |
HSP90B1 | F:CTGGGACTGGGAACTTATGAATG R:TCCATATTCGTCAAACAGACCAC | 217 |
IRE1α | F:CACAGTGACGCTTCCTGAAAC R:GCCATCATTAGGATCTGGGAGA | 169 |
PERK | F:ACGATGAGACAGAGTTGCGAC R:ATCCAAGGCAGCAATTCTCCC | 80 |
EIf2α1 | F:GCTTGCTATGGTTACGAAGGC R: CATCACATACCTGGGTGGAG | 120 |