Fig. 2

Effects on mice phenotypes after PDK1 knockout in osteoclasts. A Gross anatomy. B staining of skeletal bone tissues using Alizarin Red and Alcian Blue. C X‐ray radiographs. D Vital organs of 8-week-old PDK1 cKO and WT mice. E, F Comparison of tibia length of PDK1 cKO and WT mice. G Body weight changes of 1- to 12-week-old PDK1 cKO and WT mice. H Comparison of bone resorption markers (TRAC5b, CTX-1) and bone formation markers (P1NP, BGP) in PDK1 cKO and WT mice. N = 6, all data are expressed as the mean ± SD. ^NSP > 0.05, *P < 0.05, **P < 0.01, and ***P < 0.001. PDK1, 3-phosphoinositide-dependent protein kinase-1; WT, wild type; cKO, conditional knock out; TRACP‐5b, tartrate‐resistant acid phosphatase 5b; CTX‐1, C‐terminal telopeptide of type I collagen; PINP, procollagen I N‐terminal propeptide; BGP, bone Gla protein